High Throughput Multi Parameter TEM Chemical Processing Protocol Development with the mPrep-s Capsule System: Schmidtea mediterranea

It is widely accepted that chemical processing protocols for transmission electron microscopy (TEM) must be optimized for each different organism or tissue type for a particular research need. While processing conditions have been established for many tissues, there are some organisms and tissue types that are not well preserved with standard protocols and aren’t represented in current literature for a particular research need. This study demonstrates a high throughput strategy for multi-parameter protocol testing in one processing run using the mPrep capsule and micropipettor-based system from Microscopy Innovations [1], and a quick comparison method using a silicone micro-well insert from Ibidi [2].